Amplification of the Streptococcus faecalis proton-translocating ATPase by a decrease in cytoplasmic pH

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Amplification of the Streptococcus faecalis proton-translocating ATPase by a decrease in cytoplasmic pH.

When Streptococcus faecalis was grown in the presence of protonophores , an ATPase activity of the membrane was increased at a pH below 8.0 but not at a pH above 8.0. Characteristics of this increased ATPase were identical to those of a proton-translocating ATPase (H+-ATPase) located on the membrane of normal cells. The cytoplasmic pH was regulated at 7.6 to 7.8 but was not regulated in the pre...

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Lowering of cytoplasmic pH is essential for growth of Streptococcus faecalis at high pH.

The growth of Streptococcus faecalis at high pH was significantly stimulated by carbonate. In the absence of added carbonate the cells were unable to grow at a pH above 9.5, but in media containing 50 mM HCO3- they grew even at pH 10.5. Both rate and yield of growth at pH 9.5 were significantly stimulated by as little as 5 mM carbonate. The cytoplasmic pH in growing cells was maintained at abou...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1984

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.158.3.1157-1160.1984